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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 27, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Development of Mass Spectrometry-Based SCFA Analysis Methods in Diverse Samples for Microbiome Research.

Chaeeun Park1,2, Md Abdur Rahim2, Indrajeet Barman2,3

  • 1Department of Medical Science, Graduate School, Soonchunhyang University, 22, Soonchunhyang-Ro, Sinchang-Myeon, Asan-si 31538, Republic of Korea.

Life (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

Accurate measurement of short-chain fatty acids (SCFAs) is crucial for understanding microbiome health. Optimized headspace GC-MS and GC-MS/MS methods provide sensitive quantification across diverse biological samples.

Keywords:
GC-MS/MSbiological matricesheadspace analysismicrobiomeshort-chain fatty acids (SCFAs)

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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

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06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

Area of Science:

  • Microbiome research
  • Metabolomics
  • Analytical chemistry

Background:

  • Short-chain fatty acids (SCFAs) are key microbial metabolites influencing host physiology.
  • Accurate SCFA quantification is vital for microbiome-host interaction studies.
  • Standardized methods for SCFA measurement across various biological matrices are lacking.

Purpose of the Study:

  • To optimize and validate two analytical methods for SCFA quantification.
  • To assess the applicability of these methods to diverse biological samples.
  • To provide robust platforms for SCFA profiling in microbiome research.

Main Methods:

  • Optimization of headspace Gas Chromatography-Mass Spectrometry (GC-MS) for direct SCFA analysis.
  • Development of Gas Chromatography-tandem Mass Spectrometry (GC-MS/MS) involving extraction, alkaline treatment, and derivatization (MTBSTFA).
  • Application of both methods to microbial cultures, animal liver, feces, and simulated human fecal samples.

Main Results:

  • Headspace GC-MS offers high throughput with minimal sample preparation.
  • GC-MS/MS demonstrates superior sensitivity and precision, ideal for low-abundance samples.
  • Both methods provide reliable SCFA quantification across tested biological matrices.

Conclusions:

  • Optimized headspace GC-MS and GC-MS/MS protocols offer robust and sensitive SCFA profiling.
  • These validated methods support deeper understanding of microbiome-host interactions.
  • The developed platforms facilitate future translational applications in SCFA research.