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Toxicity and potential of propolis resin to boost embryo growth of broilers
Maslichah Mafruchati1, Lailatul Fadliyah2, Akhmad Kusuma Wardhana3
1Department of Veterinary Anatomy, Faculty of Veterinary Medicine (60115), Universitas Airlangga, Mulyorejo, Surabaya, Indonesia.
Background:
Propolis resin could be used as a therapeutic agent for chicken embryos, but its toxicity must be evaluated before application in livestock.
Aim:
This study aimed to investigate the toxicity and growth-promoting properties of propolis resin on the embryo of the chicken.
Methods:
Fertilized broiler eggs were used as experimental samples by being injected with propolis resin. The solution volume was predetermined to be 1 ml per egg for each treatment dose.
Results:
The result showed that the morphology of chicken eggs was viable after propolis resin injection on days 13-21 after inoculation. Moreover, 62.5 mg/egg was the effective dose, 125 mg/egg was the Toxic dose 250 mg/egg was the lethal dose. A mid-range dose (62.5-125 mg/egg) was associated with higher embryo weight and wing bone length compared to controls, though differences require further confirmation.
Conclusion:
Propolis resin at intermediate concentration can boost embryonic growth, but higher doses rapidly induce toxicity. These findings emphasize the importance of dose optimization and chemical profiling before in-ovo implementation. Further studies across poultry species are needed to evaluate antiviral and growth-promoting effects while ensuring safety.

