Related Experiment Video
Updated: Jul 1, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Published on: May 7, 2018
Sphingolipid diversity and profiling in selected Caryophyllales species
Hirokazu Tokimizu1, Toshiki Ishikawa2, Hiroyuki Imai1
1Department of Biology, Graduate School of Natural Sciences, Konan University, Hyogo, 658-8501 Japan.
Abstract:
Sphingolipid composition was compared among selected Caryophyllales species using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Glycosylinositol phosphorylceramide (GIPC) molecular species profiles differed between the AC group (A. philoxeroides, A. denticulata, and C. argentea) and the SB group (S. oleracea and B. vulgaris ssp. altissima), accompanied by variation in glycan series distribution and the GIPC/glucosylceramide (GlcCer) ratio. Differences in sphingoid base modification and ceramide structure were also observed, suggesting group-associated differences in sphingolipid metabolism and potential value for future chemotaxonomic studies.
More Related Videos
08:56Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
13:02Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
Related Concept Videos
Membrane Lipids
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Biosynthesis of Lipids