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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Jul 2, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Engineering Guide RNAs for CRISPR-Based Biosensors.

Xiaojing Liu1, Tingyi Chen1, Xiangjun Li1

  • 1Zhongshan School of Medicine, Sun Yat-sen University, Shenzhen, Guangdong 518107, China.

ACS Sensors
|June 30, 2026
PubMed
Summary
This summary is machine-generated.

Engineering single-guide RNAs (sgRNAs) enhances CRISPR-Cas biosensors for clinical diagnostics. Modified sgRNAs improve stability and specificity, overcoming limitations of native versions for sensitive detection.

Keywords:
CRISPR-Cas systembiosensorsclinical diagnosticsengineered sgRNAmicrofluidic biosensorsnon-canonical nucleic acid modificationspoint-of-care Testingwearable biosensors

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Related Experiment Videos

Last Updated: Jul 2, 2026

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

  • Biotechnology and Biosensing
  • Molecular Biology and Genetics

Background:

  • CRISPR-Cas systems offer programmable nucleic acid targeting for sensitive biosensor development.
  • Native single-guide RNAs (sgRNAs) present limitations including instability, immunogenicity, and off-target effects, hindering clinical translation.

Purpose of the Study:

  • To systematically review advancements in engineering single-guide RNAs (sgRNAs) for CRISPR-Cas biosensor applications.
  • To address limitations of native sgRNAs and enhance biosensor performance for clinical diagnostics.

Main Methods:

  • Review of CRISPR-Cas effector proteins, their mechanisms, and structural features.
  • Analysis of native sgRNA limitations in biosensing.
  • Examination of sgRNA engineering strategies: chemical modifications, structural remodeling, and functional integration.
  • Integration of engineered sgRNAs into diverse biosensor platforms (e.g., microfluidics, wearables, POCT).

Main Results:

  • sgRNA engineering strategies significantly improve biosensor stability, specificity, and reliability.
  • Engineered sgRNAs have been integrated into various advanced biosensor platforms.
  • Comparative analysis highlights performance metrics like detection signals and limits of detection.

Conclusions:

  • Engineering sgRNAs is crucial for overcoming native limitations and enhancing CRISPR-based biosensor performance.
  • Advances in sgRNA engineering accelerate the development of stable, specific, and reliable biosensors.
  • Future directions focus on amplification-free, multiplexed, and clinically translatable CRISPR biosensors.