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Related Experiment Video

Updated: Jul 2, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Identification of Differentially Expressed Genes in Non-functioning Pituitary Adenoma by Transcriptome Analysis.

Nisa Devrim Uzkul1, Deniz Sunnetci Akkoyunlu2, Burak Cabuk3

  • 1Department of Medical Genetics and Molecular Biology, Health Sciences Institute of Kocaeli University, Kocaeli, Türkiye; nisadevrimuzkul@gmail.com.

In Vivo (Athens, Greece)
|June 30, 2026
PubMed
Summary

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Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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This study identified key genes in non-functioning pituitary adenoma (NFPA) pathogenesis. Genes like CCND1, POU5F1, SOX2, HNF1A, BRCA1, and FOXA1 may serve as diagnostic and therapeutic targets for NFPA.

Area of Science:

  • Endocrinology
  • Oncology
  • Genomics

Background:

  • Non-functioning pituitary adenoma (NFPA) is the most common pituitary tumor, often diagnosed late due to compression symptoms.
  • Current surgical treatments for NFPA have suboptimal remission rates.
  • Understanding the molecular mechanisms of NFPA is crucial for improving patient outcomes.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) involved in the pathogenesis of NFPA.
  • To explore signaling pathways and key molecules implicated in NFPA development.
  • To discover potential biomarkers for NFPA diagnosis and targeted therapy.

Main Methods:

  • Gene expression microarrays were used to compare 45 NFPA samples with 8 normal controls.
  • Enrichment analysis was performed to identify significant signaling pathways in NFPA.
Keywords:
CCND1Non-functioning pituitary adenomacytokine-cytokine receptor interactiondifferential gene expressiongene expression microarray

Related Experiment Videos

Last Updated: Jul 2, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

  • Protein-protein interaction (PPI) networks were constructed to pinpoint key genes.

    • Main Results:

    • 270 genes were upregulated, and 2,428 genes were downregulated in NFPA samples.
    • Cytokine-cytokine receptor interaction was a significantly affected pathway, with upregulation of PRLR, TNFRSF21, and LEPR.
    • CCND1, POU5F1, SOX2, HNF1A, BRCA1, and FOXA1 emerged as critical nodes in PPI networks.

    Conclusions:

    • CCND1, POU5F1, SOX2, HNF1A, BRCA1, and FOXA1 were identified as key molecules in NFPA.
    • These genes hold potential as biomarkers for NFPA diagnosis.
    • These identified molecules may offer new avenues for targeted NFPA therapy.