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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: Jul 6, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Knockdown of Chimeric RNA by RNAi.

Liang Cheng1, XiaoLe Lu1, Chenwei Yang1

  • 1Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2026
PubMed
Summary

This study details RNA interference (RNAi) methods for studying chimeric RNAs, addressing challenges like sequence homology. It provides guidance on knockdown techniques, downstream analyses, and necessary controls for functional studies.

Keywords:
Chimeric RNAFunctional assayRNAiShort hairpin RNA (shRNA)Small interfering RNA (siRNA)

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Last Updated: Jul 6, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

Area of Science:

  • Molecular Biology
  • Gene Expression Regulation

Background:

  • RNA interference (RNAi) is a key technique for gene function studies.
  • Traditional RNAi uses short hairpin RNAs (shRNAs) or short interfering RNAs (siRNAs) to degrade target mRNA.
  • Chimeric RNAs present unique challenges for RNAi due to sequence homology limitations.

Purpose of the Study:

  • To provide a comprehensive guide for studying chimeric RNAs using RNA interference (RNAi).
  • To outline methods for effectively knocking down chimeric RNAs.
  • To detail downstream assays and essential controls for chimeric RNA functional studies.

Main Methods:

  • Utilizing RNA interference (RNAi) for targeted knockdown of chimeric RNAs.
  • Implementing specific short hairpin RNAs (shRNAs) or short interfering RNAs (siRNAs).
  • Designing and executing functional assays to assess knockdown effects.

Main Results:

  • Sequence homology at the chimeric junction can limit RNAi targeting.
  • Potential for unintended consequences on parental genes requires careful control.
  • Established protocols for effective chimeric RNA knockdown and analysis.

Conclusions:

  • RNAi can be adapted to study chimeric RNAs, despite inherent challenges.
  • Careful experimental design and appropriate controls are crucial for reliable results.
  • This chapter serves as a practical resource for researchers investigating chimeric RNA function.