Hemostatic properties of mesoporous silica nanoparticles loaded with Typhae Pollen-derived carbon dots

This study develops a multifunctional hemostatic nanocomposite through hierarchical integration of Typhae Pollen-derived carbon dots (TPC-CDs) with mesoporous silica nanoparticles (MSNs). Addressing the limitations of traditional Typhae Pollen (low bioavailability, undefined pharmacophores), the TPC-CDs@MSNs system achieves pH-modulated flavonoid release (37.6 ± 1.8% cumulative release at pH 5.5 vs. 22.8 ± 1.6% at pH 7.4 over 24 h) and exhibits good biocompatibility and antibacterial activity. Material characterization confirmed uniform MSNs morphology and successful TPC-CDs incorporation, as evidenced by TEM and FTIR analyses. In vitro coagulation assays demonstrated significant reduction in coagulation time (PT: 12.6 ± 0.5 s vs. control 20.4 ± 0.9 s; APTT: 37 ± 2.7 s vs. control 50 ± 3.5 s; p < 0.001), indicating dual activation of intrinsic/extrinsic pathways. In vivo rat tail hemorrhage models revealed rapid hemostasis and reduced blood loss. This study established an integrated phytochemistry-nanotechnology strategy to develop a versatile hemostatic material with efficient bleeding control and broad-spectrum antibacterial properties, thereby opening new avenues for carbon-dot applications in biomedical materials.