Indica Rice Anther and Shed Microspore Culture

During the last few decades, the induced mutation and selection procedure has been widely used as an efficient method for the rapid production of biotic stress-resilient plants in a short period of time. The efficiency of this method has been shown to be further enhanced when combined with the doubled haploid (DH) technology. DH technology facilitates the rapid and efficient identification of recessive and dominant mutants in the first generation at either haploid or DH levels. Additionally, it also fixes the mutation without the need for further selection, which is commonly used in the conventional mutation breeding. To be practically feasible in breeding programs, DH technology needs to be well optimized in the desired species to obtain sufficient numbers of stable DH plants in a wide range of genotypes. The protocol described here represents an efficient procedure for DH production via both anther and shed-microspore culture (SMC) techniques in O. sativa L.