Related Experiment Video
Updated: Aug 6, 2026

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Published on: April 5, 2018
Beyond salting-in: Activating myofibrillar proteins for high internal phase emulsions via a pH-ionic strength
Feiyu Zhang1, Peng Wang1, Weiyi Zhang1
1State Key Laboratory of Meat Quality Control and Cultured Meat Development, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
Myofibrillar proteins (MPs) readily aggregate in low-ionic-strength media, limiting their solubility and emulsifying performance. MPs were examined across pH 3.0-11.0 in deionized water (ultra-low ionic strength) and high-ionic-strength medium (HIS, 0.6 M KCl, 50 mM phosphate), using high internal phase emulsions (HIPEs) as a functional model. MPs showed medium-dependent pH responses: in water, pH 3.0 and 11.0 improved dispersion and supported HIPE formation, whereas in HIS, pH 3.0 caused severe precipitation and pH 11.0 yielded the clearest solutions; maximal precipitation occurred at pH 5.0 (water) and pH 3.0 (HIS). These results indicate a pH-ionic strength complementarity mechanism. Under favorable extreme-pH conditions, MPs showed reduced particle size, structural unfolding, increased hydration, lower viscosity, and rapid formation of rigid interfacial films, enabling HIPEs with semi-solid rheology and improved stability. These findings support the design of pH-tunable, low-salt meat protein colloids.
More Related Videos
09:54Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
Published on: November 19, 2015
04:25Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
Published on: August 8, 2025
Related Concept Videos
Ionic Strength: Effects on Chemical Equilibria
In this solution, the primary cation—the calcium...
Ionic Strength: Overview
Detergent Purification of Membrane Proteins
Smooth Muscle Contraction
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Relaxation of Skeletal Muscles
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
Generation of Action Potential in Skeletal Muscles
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the cell's...