Related Experiment Video
Updated: Aug 6, 2026
A Neonatal Mouse Model of Necrotizing Enterocolitis and Lamina Propria Isolation for Immune Cell Profiling
Published on: September 19, 2025
The effect of ethyl pyruvate administration on intestinal mucosal damage in neonatal rat models of necrotizing
Tugce Merve-Orbay1, Aysenur Celayir1, Hulya Yavuz2
1Department of Pediatric Surgery.
Objective:
The purpose of this study was to determine whether intraperitoneal administration of ethyl pyruvate (EP) affects intestinal mucosal damage in neonatal rats with a model of necrotizing enterocolitis (NEC).
Methods:
The study comprised 46 Sprague-Dawley newborn rats delivered on the 21st gestational day. The rats were divided into four groups: Sham Group (n = 10), EP Group (n = 10), NEC Group (n = 13), and NEC + EP Group (n = 13).
Results:
The mean body weights of rats in Sham and EP groups significantly increased (p < 0.05), whereas those in NEC groups significantly decreased (p < 0.05). The changes were similar between groups (p > 0.05). There was no significant difference in the clinical sickness score (CSS) between groups (p > 0.05). The CSS in the NEC groups showed an increase from day 1 to day 4. Based on the histopathological grading, there was a statistically significant difference (p < 0.001) observed between the Sham Group, the NEC Group, and the NEC + EP Group. The differences in biochemical values among the other groups were found to be statistically significant (p < 0.05) for all variables.
Conclusions:
The application of EP in high-mortality diseases such as NEC is expected to reduce surgical needs, establishing a basis for future studies on EP as an innovative anti-inflammatory agent.
