Related Experiment Video
Updated: Aug 6, 2026
10:34
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Genomic Full-Length Sequence of the KIR3DL3*04802 Allele Was Identified by PacBio SMRT Sequencing
1Central Laboratory, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, Guangdong, China.
HLA
|July 21, 2026
Summary
The KIR3DL3*04802 gene sequence in Han individuals was fully sequenced using PacBio technology. This specific KIR3DL3 variant differs from KIR3DL3*04801 by nine nucleotide variations.
Area of Science:
- Immunogenetics
- Human genetics
Background:
- Killer cell immunoglobulin-like receptors (KIRs) are crucial in immune regulation.
- KIR3DL3 is a gene within the KIR family with known functional importance.
Purpose of the Study:
- To fully characterize the KIR3DL3*04802 allele sequence.
- To identify genetic differences between KIR3DL3*04802 and KIR3DL3*04801.
Main Methods:
- Full-length sequencing of the KIR3DL3*04802 gene using PacBio long-read technology.
- Comparative sequence analysis.
Main Results:
- The complete genomic sequence of KIR3DL3*04802 was obtained from a Han individual.
- Nine nucleotide differences were identified between KIR3DL3*04802 and KIR3DL3*04801 sequences.
Conclusions:
- The KIR3DL3*04802 allele represents a distinct genetic variant.
- These sequence differences may have implications for KIR3DL3 function and immune response.
Related Concept Videos
01:05Maxam-Gilbert Sequencing
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
01:57Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...