Synthesis of super-biosafety hydrogen peroxide solution by ultrasonic cavitation

Hydrogen peroxide (H₂O₂) is the most widely used medical disinfectant product. However, the H₂O₂ solution synthesized by the industrial anthraquinone method currently contains trace amounts of heavy metal ions and toxic organic by-products, which pose a potential threat to human life and health. In this study, we propose the synthesis of super-biosafety H₂O₂ solution without any impurity through ultrasonic cavitation from pure water saturated with inert gases. The biosafety of H₂O₂ solution synthesized by ultrasonic cavitation (H₂O₂-U) was fully confirmed to be higher than that of commercial H₂O₂ (H₂O₂-C) by in vitro cell and in vivo mouse experiments. Computational fluid dynamic (CFD) simulation proved that the internal temperature of the argon (Ar) bubbles (710.50 K at 2.24 μs) is much higher than that of nitrogen (N₂) bubbles (569.30 K at 2.26 μs). It indicated that Ar more favors water splitting to form H₂O₂, in good agreement with the experimental results. Both radical experiment and hydrodynamic simulations disclose the reaction essence that sonication-induced local high temperature and pressure split pure water to ·OH and ·H radicals, which subsequently couple to generate H₂O₂ and H₂. This work proposes an infusive methodology for the efficient synthesis of super biosafety H₂O₂ solution, and presents the first real-life applications.