Differences between cull and normal turkeys in natural infection with Mycoplasma meleagridis at one day of age

The thoracic air sacs of poults at one day of age were examined to compare those of normal, saleable poults with those of sibs culled(1) at hatching at three stages in the laying season. Lesions of airsacculitis were recorded and the air sacs were cultured for mycoplasma. Impression smears of the thoracic air sacs were stained with rabbit anti-Mycoplasma meleagridis serum conjugated with fluorescein isothiocyanate and examined to determine the location and infection rate of the organism. M. meleagridis were observed closely associated with or within epithelial cells of the air sac; within mononuclear cells and as extracellular "microcolonies". The lesion and infection rate of cull poults in most hatches exceeded that of normal poults and infection with M. meleagridis was more often expressed (as a lesion) in cull than in normal poults.