Biphasic Interfacial Transmission Enables DNA Storage in Liquid

Area of Science:

• Biotechnology
• Data Storage
• Biochemistry

Background:

• DNA offers high-density data storage potential to combat the global data storage crisis.
• Efficient and low-loss transmission and preservation of synthetic DNA are crucial for data integrity.
• Energy consumption during DNA data handling remains a significant challenge.

Purpose of the Study:

• To establish an energy-efficient aqueous biphasic interfacial transmission (BIT) pathway for synthetic DNA.
• To enable simultaneous salt-decontaminated migration and long-term anti-aging preservation of DNA data.
• To ensure high DNA transfer efficiency and preserve DNA integrity during the process.

Main Methods:

• Development of an aqueous biphasic system utilizing specific temperature windows (20°C, 25°C, or 30-40°C) for DNA transmission.
• Implementation of a 30-minute standing solution state or a 2-minute centrifugation mode for accelerated transfer.
• Utilizing a PEG-rich preservation environment with low water activity to stabilize DNA.

Main Results:

• The BIT pathway achieved a DNA transfer efficiency exceeding 80% with an interphase partition coefficient over 264.
• Theoretically extrapolated half-life of DNA is up to 577 years at -20°C, demonstrating excellent preservation.
• The process operates efficiently at room temperatures with minimal energy input, completing in minutes.

Conclusions:

• The BIT pathway provides a scalable, energy-efficient strategy for DNA data storage circulation and preservation.
• The method effectively stabilizes nucleotide bonding and preserves DNA secondary structure information.
• This approach offers a robust solution for securing massive information in synthetic DNA.