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Thermophysical Properties of a tRNA Isodecoder
Lev Levintov1, Esteban A Orellana2,3, Harish Vashisth1,4,5,6
1Department of Chemical Engineering and Bioengineering, University of New Hampshire, Durham, New Hampshire03824, United States.
Abstract:
The heat capacity (Cp) is a fundamental thermodynamic property widely used to characterize biomolecular conformational stability. Despite extensive investigations into changes in the heat capacity (ΔCp) associated with protein folding, these effects remain incompletely characterized in the folding of ribonucleic acid (RNA) molecules. In this work, we report on the thermophysical properties of a transfer RNA (tRNA) isodecoder in its chemically unmodified form as well as two modified forms, each having a distinct chemical modification, N2,N2-dimethylguanosine (m2,2G) or N7-methylguanosine (m7G). The computed Cp profile of the unmodified tRNA displayed a well-defined peak consistent with experimentally reported Cp thermograms for various tRNAs. Based on the consistency between the computed thermophysical properties and experimental observations, we further probed the effects of different monovalent ions, distinct interatomic potentials, and varying melting temperature values on the key thermophysical properties of the tRNA isodecoder.
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