A Transferable Quantitative Framework for Extracting Engineering-Relevant Descriptors from Biological Protective...

Citrus peel is examined here as a naturally evolved protective surface, with the goal of developing a transferable quantitative framework for extracting engineering-relevant descriptors from biological protective surfaces and using them as design templates for biomimetic counterparts. A single-specimen-per-species design is adopted to map intra-fruit geometric variation across regions and magnifications; absolute descriptor values are therefore reported as ordinal indicators of inter-species ranking rather than as population means. Five citrus species (lemon, orange, mandarin, grapefruit, and bitter orange) were characterised by mechanical testing (cutting, puncture, and compression; five replicates per fruit), gravimetric peel density and thickness, and scanning electron microscopy (SEM) at 100×-10,000×. The 135-image SEM dataset was processed with an automatic-calibration pipeline performing per-image scale-bar detection, multilevel-Otsu segmentation of albedo air space, cell-bounded surface segment (CBSS) and oil-gland segmentation on flavedo, and grey-level co-occurrence matrix (GLCM) texture analysis with a directional anisotropy index AF. Calibration was consistent across all images (FoV × magnification =403,273±410 μm·×, ±0.10%). Principal component analysis separated flavedo and albedo at every magnification (PC1 + PC2 = 84-92%). Within this dataset, grapefruit showed the densest CBSS cover (1072 mm-2) together with the highest oil-gland density (2.77 mm-2); bitter orange showed the largest CBSS area (23.7 μm2) and the thickest peel (13.1 mm); mandarin showed the most directionally oriented flavedo film (AF=0.0885); and lemon showed the most open albedo (φ2D=36.2%). Oil-gland equivalent diameter was essentially invariant (∼45 μm) across the five fruits, while gland density varied 4.4-fold. The structural metrics define a layered descriptor space-a dense isotropic surface relief versus a thick cellular bulk-that supplies two distinct bioinspired-design priors: dense surface films as a structural prior for selective-permeability membranes and layered cellular cores as a prior for impact-absorbing panels. A modified-atmosphere packaging (MAP)-compatible biomimetic film is identified as one downstream design hypothesis requiring direct gas-permeability verification on synthetic membranes.