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Updated: Aug 5, 2026

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Published on: September 9, 2014
Features of IL-1β Secretion by Macrophages in Monocyte Culture in Colorectal Cancer
N K Sadykhov1, N K Shakhpazyan2, L M Mikhaleva2
1Avtsyn Research Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow, Russia. drawnman@mail.ru.
Abstract:
The level of IL1β secretion in culture of LPS-stimulated CD14+ monocytes from patients with colorectal cancer was determined. Significant differences in cytokine levels were found in patients with early (15.39 [8.358-25.52] pg/mL) and late (30.33 [16.74-44.20] pg/mL) stages of the disease (p = 0.0381). We also revealed a drastic stage-dependent increase in the response to the first stimulation: by 1.6 times in stage I and by 9 times in stage IV (p = 0.023).
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