Method-Dependent Variability in Hempseed Lipidomics: Combined Influence of Extraction, Derivatisation, and Genotype...

Area of Science:

• Agricultural Science
• Food Chemistry
• Lipidomics

Background:

• Hempseed (Cannabis sativa L.) is a rich source of polyunsaturated fatty acids (PUFA).
• Understanding the impact of extraction and derivatization on hempseed lipid profiles is vital for standardization.
• Fibre-type hemp cultivars require specific analytical considerations for lipidomic studies.

Purpose of the Study:

• To evaluate the effects of microwave-assisted and Soxhlet extraction combined with acidic and basic derivatization on hempseed lipid quality.
• To assess the fatty acid composition, lipid quality indices, and oxidative stability of Romanian fibre-type hempseed oils.
• To identify the primary sources of variability in hempseed lipidomic analysis.

Main Methods:

• Microwave-assisted extraction and Soxhlet extraction were employed.
• Acidic and basic derivatization techniques were applied to the extracted oils.
• Fatty acid composition, lipid quality indices, and oxidative stability were analyzed.

Main Results:

• Hempseed oils were rich in linoleic and α-linolenic acids, exhibiting favorable nutritional quality indices.
• Extraction and derivatization methods significantly impacted fatty acid composition and lipid quality.
• Extraction methodology was identified as the main source of variability in the analyses.

Conclusions:

• Romanian fibre-type hemp cultivars are valuable sources of nutritionally superior lipids.
• Standardized protocols for extraction and derivatization are essential for accurate and comparable hempseed lipidomic data.
• Further research into the relationship between lipid profiles and oxidative stability is warranted.