Material Properties of Human Bone-Derived Gelatin

A comprehensive evaluation of allogeneic human bone-derived gelatin (hBG) was conducted as a promising material for regenerative medicine and 3D bioprinting. Comparative analysis with a commercial animal-derived gelatin product (hereinafter CDH) revealed a similar FTIR spectral profile, confirming the retention of key functional groups. Rheological analysis under steady shear showed that hBG is a pseudoplastic fluid with a concentration-dependent viscosity. However, a complete characterization of bioinks requires oscillatory measurements (G', G″, and gelation point), which are planned in future studies. In vitro experiments on spheroid models (chondroblasts) demonstrated biocompatibility, an absence of cytotoxicity, and support for cell viability in 5% and 10% (hBG-5/10) matrices. Owing to its origin, the material allows for the modeling of "human-in-human" conditions, which makes it a promising precursor for the development of bioinks and matrices in regenerative medicine and tissue engineering. However, definitive positioning of the material as a bioink requires additional oscillatory rheological studies (determination of G', G″, thixotropy, and gelation point), which were not performed in the present work.