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Updated: Aug 5, 2026

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Published on: May 1, 2017
PEPPI-SP3: A Gel-Based High-Resolution Sample Preparation Workflow for In-Depth Top-Down Analysis of Intact
Ayako Takemori1, Jake T Kline2, Luca Fornelli2,3
1Advanced Research Support Center, Ehime University, Toon, Ehime, 791-0295, Japan.
Abstract:
Top-down proteomics enables large-scale identification of proteoforms by analyzing intact proteins extracted from biological samples using mass spectrometry systems coupled with liquid chromatography or capillary electrophoresis. In this chapter, we present the PEPPI-SP3 workflow, a high-resolution sample prefractionation method that significantly enhances the analytical depth of top-down proteomics by combining highly efficient passive extraction of in-gel proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis with SP3 bead-based purification of intact proteins.
