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Published on: August 25, 2018
[Evaluating the taxonomy of Lacticaseibacillus casei in National Center for Biotechnology Information genome database
Qi Wang1, Lifeng Yang2, Wei Wang3
1Science and Technology Research Center of China Customs, Beijing 100026, China.
Objective:
Reverificate the taxonomic information of Lacticaseibacillus casei in the genome database of National Center for Biotechnology Information (NCBI) to afford accurate genomic information for L. casei species-specific sequence analysis.
Methods:
Based on the whole genome sequence downloaded from NCBI database, the taxonomic information of 45 L. casei was reverificated by average nucleotide identity (ANI) analysis and phylogenetic analysis based on 16S rRNA gene and 49 core genes.
Results:
No 16S rRNA gene sequence was extracted from 4 genomes. Of the remaining 41 genomes, the analysis results of ANI were highly in accordance with that of core gene phylogenetic analysis. The results were 21 genomes identified as L. casei and 20 genomes as L. casei related species. Using 16S rRNA gene alone could not accurately identify L. casei and its related species.
Conclusion:
It is necessary to reverificate the taxonomic information of L. casei in NCBI genome database before using its genome information for further analysis.
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