Hank's Balanced Salt Solution Supplemented With L-Ascorbic Acid Modulates Periodontal Ligament Cell Inflammation and...

Area of Science:

• Biomaterials Science
• Dental Research
• Tissue Engineering

Background:

• Preserving periodontal ligament viability is crucial for avulsed tooth management.
• Hank's Balanced Salt Solution (HBSS) lacks bioactive components for active tissue repair.
• L-ascorbic acid (L-AA) was investigated for its potential to enhance periodontal healing.

Purpose of the Study:

• To evaluate the effect of L-ascorbic acid (L-AA) supplementation in HBSS on periodontal ligament cells.
• To assess L-AA's impact on wound closure, inflammation, and extracellular matrix expression.
• To determine if L-AA enhances periodontal ligament tissue viability and gene expression in vitro and ex vivo.

Main Methods:

• Utilized in vitro (human periodontal ligament cells) and ex vivo (premolar teeth) models.
• Cells and teeth were exposed to HBSS, HBSS with L-AA (175 μg/mL), or DMEM with FBS.
• Assessed wound closure, cytokine and matrix metalloproteinase expression, and collagen I expression.

Main Results:

• L-AA supplementation significantly improved wound closure compared to HBSS alone.
• L-AA significantly reduced pro-inflammatory cytokine and matrix metalloproteinase expression.
• L-AA enhanced collagen type I alpha 1 chain expression in both models; tissue viability showed non-significant improvement.

Conclusions:

• L-ascorbic acid supplementation favorably modulated inflammatory and ECM responses in periodontal ligament tissues.
• Findings suggest potential biological benefits for periodontal repair, despite no significant increase in tissue viability.
• Further research in animal models is needed to confirm clinical relevance for avulsed tooth management.