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Updated: Aug 14, 2026
In vivo Optogenetic Stimulation of the Rodent Central Nervous System
Published on: January 15, 2015
Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics
Glena Travis1,2, Hallur Reynisson3, Anai Gonzalez-Cordero4,5
1Institute for Photonics and Optical Sciences (IPOS), School of Physics, The University of Sydney, Camperdown, NSW, Australia.
Abstract:
Bidirectional optogenetics enables simultaneous optical readout and manipulation of neural activity. However, optical crosstalk between actuators and indicators impedes clean observation and control. This review surveys genetically encoded indicators and optogenetic actuators, and the mechanisms of crosstalk, then catalogues reported crosstalk-free pairings and trade-offs. Finally, we propose best-practice guidelines and identify future directions, including near-infra-red and two-photon modalities, protein engineering and computational correction.
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