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Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
A Novel Approach for Reducing Enteropathogens Using LAB-Fermented Whey Solution and UV-C Light-Activated Gallic Acid
Luz Alejandra Márquez-Martínez1, Xochitl Aparicio-Fernández2, Genaro Gustavo Amador-Espejo3
1Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Av. San Claudio y 18 Sur Ciudad Universitaria, Puebla 72570, Puebla, Mexico.
Foods (Basel, Switzerland)
|August 13, 2026
Summary
Lactic acid bacteria (LAB) fermented whey showed antibacterial activity against pathogens. However, combining LAB-fermented medium with UV-C activated gallic acid (AGA) negated this effect during gastrointestinal simulation.
Area of Science:
- Food Microbiology
- Antimicrobial Agents
- Gastrointestinal Simulation
Background:
- Enteropathogens like *Escherichia coli* and *S.* Typhimurium pose significant health risks.
- Lactic acid bacteria (LAB) and gallic acid (GA) show potential antimicrobial properties.
- UV-C light activation enhances gallic acid's antimicrobial efficacy.
Purpose of the Study:
- To evaluate the combined antibacterial activity (AA) of LAB and UV-C light-activated gallic acid (AGA) against *E. coli* and *S.* Typhimurium.
- To assess microbial survival during in vitro gastrointestinal simulation (GS).
- To determine the impact of LAB-fermented medium (LABF) and AGA on enteropathogen viability.
Main Methods:
- LAB strains were selected for fermenting soy and whey concentrates.
- LAB-fermented media (LABF) were prepared and tested for AA.
- The combined effect of LABF and AGA was evaluated during simulated gastrointestinal conditions.
Main Results:
- Whey concentrate supported the highest LAB co-culture growth (>107 CFU/mL).
- LABF demonstrated dose-dependent AA, achieving >2-log reductions against enteropathogens.
- Combining LABF with AGA resulted in null antimicrobial activity and complete microbial recovery after 24h GS.
Conclusions:
- LABF exhibits significant antibacterial activity against common enteropathogens.
- The combination of LABF and AGA loses antimicrobial efficacy during gastrointestinal transit.
- Further research is needed to optimize the application of LABF and AGA in simulated gastrointestinal systems to preserve antibacterial activity.
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