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Updated: Sep 3, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Detection of MicroRNAs Released from Argonautes
Ji Won Lee1, Chang Hoon Shin2, J Grayson Evans3
1Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Gangneung Campus, Gangneung, South Korea.
Abstract:
The Argonaute (AGO) family of proteins is fundamental to microRNA (miRNA)-mediated gene silencing, serving as the central component of the RNA-induced silencing complex (RISC) by forming direct and stable binding with mature miRNAs. While miRNA-AGO interactions are essential for controlling gene expression and closely linked to human disease, AGO-free mature miRNAs also contribute to fine-tuning gene expression regulation through direct interaction with RNA-binding proteins (RBPs). This chapter presents a detailed biochemical methodology to quantify single-stranded mature miRNAs dissociated from AGO2, providing an approach to studying miRNAs release mechanisms.
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