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Published on: August 8, 2025
Isolation, Purification, and Characterization of Leptophages
Olivier Schiettekatte1,2, Pascale Bourhy3
1Institut Pasteur, Biology of Spirochetes Unit, Paris, France.
Abstract:
Only the three leptophages LE1, LE3, and LE4 or bacteriophages of leptospires, are known to date. However, numerous prophages have been found in this genus, particularly in the genomes of pathogenic species. Consequently, some laboratories attempt to isolate leptophage particles from environmental samples or from bacterial cultures following mitomycin C induction. Here, we present several procedures for isolating, purifying, and characterizing bacteriophages based on the leptophages characterization protocols.
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