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Label-free colorimetric immuno-RCA with Tyndall effect readout

Jiarui Li1, Wenli Zhu1,2, Kaxi Chen1

  • 1Guangxi Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, 12 Jiangan Road, Guilin, 541004, P.R. China. wyzhu@glut.edu.cn.

Chemical Communications (Cambridge, England)
|September 4, 2026
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.