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Characterization of PAPP-A Patterns in Chronic Rhinosinusitis
Afaf Ennahal1, Olaf Wendler1, Vanessa Vivien Pesold1
1Department of Otolaryngology, Head and Neck Surgery, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Purpose:
High-level expression of pappalysin-A/insulin-like growth factor binding protein/insulin-like growth factor-1 (PAPP-A/IGFBP-4/5/IGF-1 axis) in Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) was previously demonstrated by our group. PAPP-A plays a key role in inflammatory diseases and cancer. The aim of this study was to localize PAPP-A in Chronic Rhinosinusitis (CRS), within tissue, inflammatory and different primary cells.
Patients And Methods:
PAPP-A was localized by immunohistochemistry (IHC) in tissues of CRSwNP (n=41), CRSsNP patients (n=12) as well as in controls (n=12). The data of eosinophil abundance in CRSwNP tissues was obtained from routine pathology. ELISA was used to quantify PAPP-A and ECP non-invasively in CRSwNP mucus (n=67). On a cellular level, PAPP-A was localized by immunofluorescence (IF) in immune and primary cells from CRSwNP (n=18).
Results:
IHC revealed three distribution patterns of PAPP-A in CRSwNP correlated to the eosinophil abundance. Moreover, IF of different immune cells confirmed the high PAPP-A synthesis by eosinophils, in addition to unspecified subpopulation of T-cells and moderately in mast cells. Besides, a vesicular distribution of PAPP-A in the cytoplasm and the membrane of epithelial and fibroblast cells was shown. Finally, ECP and PAPP-A proved a significant correlation in CRSwNP mucus.
Conclusion:
Our work is the first to illustrate the three distribution patterns of PAPP-A in CRSwNP. This underlines the potential inflammatory role of PAPP-A, particularly in CRSwNP. Targeting the PAPP-A/IGFBP/IGF-1-axis may be a new therapeutic approach for CRSwNP with high eosinophil abundance.
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