The Versatility of Pomegranate: From Phytochemical Diversity to Antimicrobial and Translational Applications

Pomegranate is increasingly being recognized as a versatile source of bioactive compounds with antimicrobial, antioxidant, anti-inflammatory, and microbiota-modulating potential. Beyond the edible arils and juice, peel, seeds, leaves, flowers, and other agro-industrial by-products are rich in ellagitannins, particularly punicalagins, as well as ellagic acid, gallic acid, flavonoids, anthocyanins, fatty acids, and related phytochemicals. Their recovery depends strongly on plant fraction, cultivar, solvent, and extraction technology, including conventional hydroalcoholic extraction, ultrasound- and microwave-assisted processes, high-pressure treatments, and enzyme-assisted methods. Pomegranate-derived preparations exhibit activity against Gram-positive and Gram-negative bacteria, fungi, multidrug-resistant pathogens, and microbial biofilms, while selected compounds may enhance the efficacy of conventional antimicrobials. Emerging evidence also indicates bidirectional interactions with microbial communities, including microbial biotransformation of ellagitannins into urolithins and modulation of beneficial taxa and microbial metabolites. This narrative review integrates agronomic and phytochemical diversity, extraction and standardization strategies, mechanisms of antimicrobial action, synergistic interactions, microbiota-related effects, and applications in food preservation, biomedicine, animal nutrition, agriculture, and environmental sustainability. Key translational limitations include compositional variability, methodological heterogeneity, insufficient standardization, limited in vivo validation, and scarce evidence from realistic application models and clinical studies. Addressing these gaps is essential for developing safe, reproducible, and scalable pomegranate-derived preparations.