Transcriptomic Features of Thyroid Signaling Disorders in Experimental Pulmonary Arterial Hypertension

Pulmonary arterial hypertension (PAH) is characterized by a neurohumoral imbalance. We studied the effect of PAH on transcriptional activity in pulmonary artery preparations. In rats with PAH induced by monocrotaline (60 mg/kg), pulmonary artery preparations were isolated and whole-transcriptome RNA sequencing was performed. The enrichment score of gene collections from the Gene Ontology and KEGG databases was determined. Significant gene sets were grouped into clusters, and genes exhibiting the greatest changes in expression and representation within these clusters were identified. The representation of functional domains in protein sequences was characterized for the key genes. PAH led to a significant increase in the enrichment score of pathways associated with contractile elements and the cytoskeleton of muscle cells (including cardiomyocytes) and a decrease in the score for pathways associated with the synthesis of ribosomal components. Clustering of the pathways revealed changes in gene expression indicative of impaired thyroid signaling.