Application of Raman Spectroscopy for Selecting Seminal Plasma Donors

The composition of seminal plasma from men with varying ejaculate parameters was analyzed using Raman spectroscopy. Distinct differences were identified in the Raman spectra of fertile men and infertile patients, particularly in amino acids, phospholipids, and saccharides. A comparative analysis of seminal plasma biomolecules was performed within the group of fertile men who were potential seminal plasma donors. It was found that the seminal plasma from certain fertile donors exhibits a similar spectral profile and has a positive effect on the morphofunctional characteristics of spermatozoa. These findings support the use of Raman spectral signatures for the selection of seminal plasma donors and the refinement of donor selection criteria.