Related Experiment Video
Updated: Oct 15, 2026
Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
Published on: January 25, 2018
Chronic, Low-Frequency Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells
Eili Tranheim Kase1, Nataša Nikolić2
1Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, Norway.
Abstract:
Electrical pulse stimulation (EPS) represents a useful in vitro model of exercise in skeletal muscle cells. By modifying stimulation patterns, e.g., voltage, frequency, pulse duration, and total stimulation time, it is possible to generate models that simulate different types of in vivo exercise under controlled conditions. Currently, there is a wide variability among existing protocols. Here, we describe a chronic, low-frequency protocol for EPS of human myotubes in 6-well plates using single bipolar pulses of 2 ms, 1 Hz at 10 V for 24 h.
More Related Videos
08:38Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
Published on: March 19, 2013
11:41Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
Published on: June 16, 2022
