Wound Healing Assay for Melanoma Cell Migration

Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. The wound healing assay is a simple, nonexpensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.