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Related Experiment Videos

Method for determining thiocyanate in serum and urine.

P Lundquist, J Mårtensson, B Sörbo

    Clinical Chemistry
    |May 1, 1979
    PubMed
    Summary

    This study presents a fast and accurate method for measuring thiocyanate in serum and urine using anion-exchange chromatography and a modified König reaction. The new assay demonstrates high precision and quantitative recovery, offering a reliable alternative for clinical diagnostics.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Accurate measurement of thiocyanate (SCN-) is crucial for clinical diagnostics and toxicological assessments.
    • Existing methods for thiocyanate quantification may suffer from interference or lack specificity.
    • A need exists for a rapid, specific, and reliable assay for thiocyanate in biological samples.

    Purpose of the Study:

    • To develop and validate a rapid and specific method for the quantification of thiocyanate in human serum and urine.
    • To assess the precision, accuracy, and potential interferences of the proposed assay.
    • To compare the performance of the new method with an established thiocyanate measurement technique.

    Main Methods:

    • Thiocyanate was isolated from biological matrices using an anion-exchange resin with selective affinity.

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  • Eluted thiocyanate was quantified via a modified König reaction utilizing sodium hypochlorite as the chlorinating agent.
  • Interference from cyanide and certain antibiotics was addressed through additional washing steps.

    • Main Results:

    • The method achieved quantitative analytical recovery of thiocyanate in both serum and urine samples.
    • High precision was demonstrated, with a coefficient of variation of 2.3% for both within-day and between-day measurements.
    • The proposed method showed satisfactory agreement when compared to an alternative assay based on thiocyanate oxidation to cyanide.

    Conclusions:

    • The developed method provides a rapid, specific, and precise means for measuring thiocyanate in serum and urine.
    • The assay is robust, with potential interferences effectively managed through procedural modifications.
    • This technique offers a valuable tool for clinical laboratories requiring accurate thiocyanate level determination.