Evidence for natural antibodies (IgG) to polyamines in human sera

Human sera contain IgGs that react with latex-putrescine spheres. Identification of the IgGs has been achieved by polyacrylamide gel electrophoresis and their reaction with peroxidase-labeled antihuman gamma-chain-specific antibodies. The inhibition of IgG fixation to latex-putrescine spheres by free spermine and putrescine provides evidence that these IgGs are specific for polyamines.