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Cell-associated proteases affect tumour cell migration in vitro.

J Varani, W Orr, P A Ward

    Journal of Cell Science
    |April 1, 1979
    PubMed
    Summary
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    Mouse fibrosarcoma cell migration is inhibited by human serum due to a cell-associated factor. Protease inhibitors neutralize this factor, restoring migratory activity in human serum.

    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Cell migration is crucial for various biological processes.
    • Serum composition significantly influences cell behavior, including migration.
    • Foetal calf serum (FCS) and human serum (HS) differ in protease inhibitor content.

    Purpose of the Study:

    • To investigate the in vitro migratory activity of mouse fibrosarcoma cells in media supplemented with FCS and HS.
    • To determine the role of protease inhibitors in modulating cell migration.
    • To identify potential cell-associated factors affecting migration in HS.

    Main Methods:

    • Assessing fibrosarcoma cell migration in vitro using media with FCS and HS.
    • Supplementing HS with various protease inhibitors (e.g., soybean trypsin inhibitor) and evaluating migration.

    Related Experiment Videos

  • Testing the effect of epsilon-amino-n-caproic acid and plasminogen depletion on migration in HS.
  • Isolating and comparing the migratory activity of a variant cell population with altered protease activity.

    • Main Results:

    • Fibrosarcoma cells exhibited active migration in FCS-containing medium but significantly inhibited migration in HS-containing medium.
    • Addition of protease inhibitors with antitrypsin activity to HS restored cell migration.
    • Epsilon-amino-n-caproic acid and plasminogen depletion did not enhance migration in HS; epsilon-amino-n-caproic acid inhibited it.
    • A variant cell population with elevated caseinolytic and chymotrypsin-like activity showed reduced migration in both FCS and HS, particularly HS.

    Conclusions:

    • A cell-associated factor interferes with fibrosarcoma cell migration in HS.
    • This inhibitory factor is likely neutralized by protease inhibitors present in FCS or added exogenously.
    • The findings suggest a complex interplay between serum proteases, inhibitors, and cell-associated factors in regulating cell migration.