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Polynucleotides associated with histone preparations
The Biochemical Journal
|March 1, 1973
Summary
Histone preparations from various sources contain small amounts of ribonucleotides and deoxyribonucleotides. Ion-exchange chromatography effectively removes these contaminants, indicating they are not uniquely complexed with histones.
Area of Science:
- Biochemistry
- Molecular Biology
- Chromatography
Background:
- Histones are crucial proteins involved in DNA packaging.
- Nucleotides are fundamental building blocks of nucleic acids.
- Understanding the purity of histone preparations is vital for accurate biochemical studies.
Purpose of the Study:
- To investigate the presence and nature of nucleotide contaminants in histone fractions.
- To assess the effectiveness of purification methods for histone preparations.
Main Methods:
- Histone fractions were prepared using acid extraction and salt dissociation methods.
- Nucleotide content was analyzed in the prepared histone fractions.
- Ion-exchange chromatography was employed to purify histone preparations.
Main Results:
- Small quantities of ribo- and deoxyribonucleotides were detected in all histone preparations.
- These nucleotides were found to be small and heterogeneous.
- Ion-exchange chromatography efficiently removed the nucleotide contaminants.
Conclusions:
- The detected nucleotides in histone preparations are likely contaminants, not uniquely bound.
- Standard purification techniques like ion-exchange chromatography are effective in removing these contaminants.
- Ensuring the purity of histone preparations is essential for downstream applications.