Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement-dependent B-cell activation by cobra venom factor and other mitogens?

P Dukor, G Schumann, R H Gisler

    The Journal of Experimental Medicine
    |February 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Analysis of archaeal core ether lipids using Time of Flight-Secondary Ion Mass Spectrometry (ToF-SIMS): Exploring a new prospect for the study of biomarkers in geobiology.

    Geobiology·2022
    Same author

    Publisher Correction: Brain charts for the human lifespan.

    Nature·2022
    Same author

    Brain charts for the human lifespan.

    Nature·2022
    Same author

    Parental education and youth suicidal behaviours: a systematic review and meta-analysis.

    Epidemiology and psychiatric sciences·2022
    Same author

    [Teaching during the COVID-19 pandemic: how do medical students evaluate interactive video-based distance bedside teaching in otorhinolaryngology?]

    HNO·2021
    Same author

    Adverse childhood experiences and substance misuse in young people in India: results from the multisite cVEDA cohort.

    BMC public health·2021
    Same journal

    Regulation of solid tumors by the peripheral nervous system.

    The Journal of experimental medicine·2026
    Same journal

    Friend or foe? IFNλ drives fibrosis.

    The Journal of experimental medicine·2026
    Same journal

    BAd-looking or CHarming: The two faces of BACH2.

    The Journal of experimental medicine·2026
    Same journal

    Bridging veins govern discrepancy of CSF efflux into dura mater in mice.

    The Journal of experimental medicine·2026
    Same journal

    Chimeric allergen receptor regulatory T cells suppress birch pollen allergic airway inflammation.

    The Journal of experimental medicine·2026
    Same journal

    Interferon-λ drives renal fibrosis by coordinating epithelial-fibroblast crosstalk.

    The Journal of experimental medicine·2026
    See all related articles

    Cobra venom factor activates both T and B lymphocytes, acting as a T-cell substitute for antibody formation. This potent inducer highlights the crucial role of complement C3 in B-cell activation and proliferation.

    Area of Science:

    • Immunology
    • Complement System
    • Cell Signaling

    Background:

    • B-cell activation requires two signals: antigen/mitogen binding and activated complement C3 interaction.
    • B-cell mitogens and T-independent antigens activate the complement system's alternative pathway.
    • The role of complement C3 in B-cell activation warrants further investigation.

    Purpose of the Study:

    • To investigate the effect of cobra venom factor (CVF) on B-cell activation.
    • To elucidate the role of complement C3 in B-cell responses.
    • To determine if CVF can substitute for T cells in antibody formation.

    Main Methods:

    • Administered purified cobra venom factor to mouse lymphocytes (T and B cells).
    • Assessed antibody formation in B-cell cultures supplemented with macrophages or 2-mercaptoethanol.

    Related Experiment Videos

  • Investigated the effect of CVF and serum on B-cell rosette formation and mitogen reactivity.

    • Main Results:

    • Cobra venom factor demonstrated mitogenic activity for both T and B lymphocytes.
    • CVF substituted for T cells, restoring antibody formation in B-cell cultures.
    • B-cell proliferation induced by mitogens and CVF depended on the availability of exogenous C3.

    Conclusions:

    • Cobra venom factor is a potent inducer of B-cell activation, potentially by providing activated C3.
    • Complement C3 is essential for T-cell-dependent and T-cell-independent B-cell activation and proliferation.
    • CVF can serve as a valuable tool for studying B-cell activation pathways and the complement system.