A rapid method for the quantitation of haemoglobin A2

Area of Science:

• Clinical Chemistry
• Hematology

Background:

• Accurate measurement of haemoglobin A2 (HbA2) is crucial for diagnosing certain anemias.
• Existing methods can be time-consuming or expensive.

Purpose of the Study:

• To develop a rapid, accurate, and inexpensive technique for HbA2 measurement.

Main Methods:

• A novel plastic device with a miniature chromatographic column was utilized.
• The device was processed with whole blood and buffers at pH 8.25 and 7.0, followed by centrifugation.
• Eluates were analyzed spectrophotometrically at 414 nm to determine HbA2 percentage.

Main Results:

• The developed technique provides rapid and accurate HbA2 quantification.
• Results obtained using this method are comparable to established standard techniques.
• The procedure is cost-effective and utilizes a simple plastic device.

Conclusions:

• This new method offers a practical and efficient approach for HbA2 determination.
• It has the potential to improve diagnostic accessibility in clinical settings.
• The technique is suitable for routine laboratory use.