Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple micromethod for 25-hydroxyvitamin D estimation.

A Fairney, C Turner, S Hanson

    Annals of Clinical Biochemistry
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Paxillin is crucial for thymus and parathyroid development by regulating the architecture of the third pharyngeal pouch endoderm.

    Cellular and molecular life sciences : CMLS·2026
    Same author

    Clinical challenges and proposed solutions for patients with invasive lobular breast cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2025
    Same author

    A core outcome set for cranioplasty following stroke or traumatic brain injury - The COAST study.

    Brain & spine·2025
    Same author

    Early postnatal hepatocyte transplantation in a child with molybdenum cofactor deficiency type B.

    Molecular genetics and metabolism·2025
    Same author

    Explorations of strategies for inclusion for newly qualified physiotherapists from racially minoritised groups in a large, urban NHS Trust, UK.

    Physiotherapy·2024
    Same author

    COVID-19 associated with universities in England, October 2020-February 2022.

    Public health·2023
    Same journal

    Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

    Annals of clinical biochemistry·2026
    Same journal

    From interpretable NT-proBNP prediction to equitable laboratory stewardship.

    Annals of clinical biochemistry·2026
    Same journal

    Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

    Annals of clinical biochemistry·2026
    Same journal

    Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

    Annals of clinical biochemistry·2026
    Same journal

    Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

    Annals of clinical biochemistry·2026
    Same journal

    Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

    Annals of clinical biochemistry·2026
    See all related articles

    A new, rapid method accurately measures 25-hydroxyvitamin D using competitive protein-binding assay. This technique is ideal for routine clinical labs needing to assess vitamin D levels and related calcium disorders.

    Area of Science:

    • Clinical Biochemistry
    • Endocrinology
    • Analytical Chemistry

    Background:

    • Accurate measurement of 25-hydroxyvitamin D is crucial for diagnosing vitamin D deficiency and related metabolic bone diseases.
    • Existing methods for vitamin D assessment can be time-consuming and require specialized equipment.

    Purpose of the Study:

    • To describe a quick, simple, and reliable method for quantifying 25-hydroxyvitamin D in serum.
    • To evaluate the suitability of this assay for routine clinical laboratory use.

    Main Methods:

    • The described method involves dichloromethane extraction of serum.
    • A competitive protein-binding assay is employed, eliminating the need for chromatography.
    • The assay requires only 100 microliters of serum per sample.

    Related Experiment Videos

    Main Results:

    • The method provides a rapid estimation of 25-hydroxyvitamin D.
    • It allows for the analysis of up to 50 samples within a single working day.
    • The assay demonstrates simplicity, speed, and a small sample volume requirement.

    Conclusions:

    • This assay is highly suitable for routine clinical biochemistry laboratories due to its efficiency and ease of use.
    • The method is particularly valuable for investigating calcium disorders linked to vitamin D metabolism abnormalities.