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Dynamic morphology applied to human and animal leukemia cells
Israel Journal of Medical Sciences
|August 1, 1979
Summary
Dynamic morphology, using scanning electron microscopy and microcinematography, helps classify white blood cell types. However, it cannot distinguish between benign and malignant cells or differentiate certain polarized blast cells.
Area of Science:
- Cell Biology
- Hematology
- Microscopy Techniques
Background:
- Dynamic morphology integrates cell shape with live-state behavior.
- It combines scanning electron microscopy (SEM) and microcinematography.
- Understanding white blood cell morphology is crucial for hematological studies.
Purpose of the Study:
- To evaluate the utility of dynamic morphology in classifying leukemic cells.
- To determine the limitations of this technique in cell identification.
- To investigate white blood cell behavior on a substrate.
Main Methods:
- Concurrent use of scanning electron microscopy (SEM) and microcinematography.
- Observation of leukemic cell behavior and surface architecture.
- Analysis of cell shape (spherical, round, polarized) and classification.
Main Results:
- White blood cells remain spherical on glass, either round or polarized, not flattening.
- Polarized cells can be classified into specific types (blasts, promyelocytes, myelocytes, granulocytes, lymphocytes).
- Round cells of similar size and polarized blast cells from different origins are not distinguishable.
Conclusions:
- Dynamic morphology is useful for classifying certain white blood cell types based on their polarized state.
- The technique has limitations in differentiating cell origins and distinguishing benign from malignant cells.
- Further methods are needed for comprehensive classification of leukemic cells.