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Host-bacteriophage interaction in Agrobacterium tumefaciens. 3. Phage-coded endolysins
Journal of Virology
|April 1, 1974
Summary
Bacteriophage endolysins were identified in Agrobacterium tumefaciens strains following phage infection or mitomycin C induction. These findings reveal endolysin presence in various Agrobacterium tumefaciens contexts.
Area of Science:
- Microbiology
- Molecular Biology
- Bacteriology
Background:
- Bacteriophages are viruses that infect bacteria.
- Endolysins are phage-encoded enzymes crucial for releasing progeny virions.
- Agrobacterium tumefaciens is a plant pathogen known to harbor phages.
Purpose of the Study:
- To detect and characterize endolysins in Agrobacterium tumefaciens.
- To investigate the presence of endolysins following phage infection and prophage induction.
Main Methods:
- Bacterial culture and infection with bacteriophage LV-1.
- Induction of prophages using mitomycin C.
- Detection and analysis of endolysin activity.
Main Results:
- Endolysins were detected in a sensitive Agrobacterium tumefaciens strain (B6) after infection with phage LV-1.
- Similar endolysins were found in induced lysogens of Agrobacterium tumefaciens (V-1 and C-58).
- A defective prophage was inferred in Agrobacterium tumefaciens C-58 based on endolysin detection.
Conclusions:
- Endolysins are present in Agrobacterium tumefaciens under different conditions.
- Phage infection and prophage induction are triggers for endolysin production in these bacteria.
- The study provides evidence for endolysin activity in Agrobacterium tumefaciens, contributing to phage biology understanding.