A semi-automated Bratton & Marshall micromethod for determining acetylator phenotype of rabbits using the Abbott...

A micromethod for the determination of acetylator phenotype using the method of Bratton & Marshall (1939) J. Biol. Chem. 128, 537--550) was adapted to the Abbott Bichromatic Analyzer (ABA-100). Advantages of this procedure include: (a) the requirement for small blood samples (20 microliter) making it a suitable pediatric method and eliminating the need for venipunctures; (b) elimination of tedious manual reading of absorbances of samples and mathematical manipulation of data by the automated capabilities of the ABA-100; (c) an increase in the accuracy of the assay by reading samples at two wavelengths thereby correcting for differences in background.