Quantitative separation of antigen-specific murine antibodies by anti-allotype chromatography

The use of Sepharose-conjugated murine anti-Iga or anti-Igb allo-antisera allowed the quantitative separation of immunoglobulins of the two allotypes. After fractionation of mixtures of anti-(T,G)-A--L antisera obtained from congenic strains differing in immunoglobulin allotype, it was possible to measure the antigen-binding capacity of specific anti-(T,G)-A--L antibodies in each allotype fraction. Analysis of artificial mixtures of immune sera obtained from homozygous Iga and Igb animals showed that this method is quantitative and internally consistent. This method of affinity chromatography was used in the analysis of specific anti (T,G)-A--L antisera from tetraparental mice.