Related Experiment Videos
Properties of bispecific rosette-forming cells. II. --Rosette formation by "educated" T-lymphocytes
Normal thymus cells transferred into thymectomized and lethally irradiated syngeneic mice responded to stimulation with sheep erythrocytes by rosette formation with these erythrocytes. This response reached a peak on the 7th day and was not associated with any production of circulating antibodies. Rosettes produced by "educated" T-cells were inhibited by anti-theta serum as well as by anti-mouse Fab serum. Simultaneous stimulation of transferred thymocytes with sheep and pigeon erythrocytes provoked the appearance of a minority of cells simultaneously binding both types of erythrocytes. Depletion of B cells contaminating normal thymocyte populatins after passage through anti-mouse Ig coated columns before their transfer in thymectomized and irradiated recipients did not prevent the appearance of simple and double RFC. Moreover, when normal thymocytes or T-cells "educated" by allogeneic stimulation were incubated at 4 degrees C with anti-SRBC and anti-PRBC mouse sera a subsequent incubation at 37 degress of resulted in the dissociation of most passive rosettes formed at 4 degrees. Conversely similar incubation at 37 degrees of rosettes formed by actively immunized cells resulted in capping of about 50% simple and double rosettes. This redistribution of membrane receptors is proposed as a routine test for distinguishing active from passive rosettes.
Normal thymus cells transferred into thymectomized and lethally irradiated syngeneic mice responded to stimulation with sheep erythrocytes by rosette formation with these erythrocytes. This response reached a peak on the 7th day and was not associated with any production of circulating antibodies. Rosettes produced by "educated" T-cells were inhibited by anti-theta serum as well as by anti-mouse Fab serum. Simultaneous stimulation of transferred thymocytes with sheep and pigeon erythrocytes provoked the appearance of a minority of cells simultaneously binding both types of erythrocytes. Depletion of B cells contaminating normal thymocyte populatins after passage through anti-mouse Ig coated columns before their transfer in thymectomized and irradiated recipients did not prevent the appearance of simple and double RFC. Moreover, when normal thymocytes or T-cells "educated" by allogeneic stimulation were incubated at 4 degrees C with anti-SRBC and anti-PRBC mouse sera a subsequent incubation at 37 degress of resulted in the dissociation of most passive rosettes formed at 4 degrees. Conversely similar incubation at 37 degrees of rosettes formed by actively immunized cells resulted in capping of about 50% simple and double rosettes. This redistribution of membrane receptors is proposed as a routine test for distinguishing active from passive rosettes.