Related Experiment Video
Updated: Aug 10, 2026
12:01
Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis
Published on: August 2, 2013
A rapid and simplified staining of juxtaglomerular granules with aqueous crystal violet
Microscopica Acta
|November 1, 1975
Abstract:
The method described allows staining of juxtaglomerular granules in a deep purple colour against faint purple background and deep purple erythrocytes. Paraffin sections of mouse kidney fixed in 10% formalin buffered with phosphate are brought to water, stained 3 min in 0.01% crystal violet, rinsed in tap water (or stained 3 min in 0.1% crystal violet in NaHCO3, rinsed in 1% NaHCO3), blotted, differentiated in a 1 : 1 aniline-xylene mixture, cleared in xylene and mounted in a resin.
Related Concept Videos
01:03Fixation and Sectioning
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
01:24Simple Staining Technique
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...