Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Trypsin as a catalyst for peptide synthesis.

T Oka, K Morihara

    Journal of Biochemistry
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Trypsin can efficiently synthesize peptides using specific conditions. Optimizing enzyme concentration, pH, and substrate concentration enhances peptide synthesis yields.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Systemic lupus erythematosus following vaccination against 2009 influenza A (H1N1).

    Lupus·2010
    Same author

    Granulomatous mycosis fungoides presenting as poikiloderma.

    Clinical and experimental dermatology·2008
    Same author

    Atypical form of necrobiosis lipoidica associated with lymphoedema.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2007
    Same author

    Follicular lupus erythematosus: a new cutaneous manifestation of systemic lupus erythematosus.

    The British journal of dermatology·2002
    Same author

    Use of Bacillus brevis for synthesis and secretion of Des-B30 single-chain human insulin precursor.

    Bioscience, biotechnology, and biochemistry·2000
    Same author

    Distribution of sarcophytol A in soft coral of the Sarcophyton genus.

    Bioscience, biotechnology, and biochemistry·2000
    Same journal

    Estrogen-related receptor γ functions as a novel corepressor of androgen receptor by destabilizing receptor conformation and blocking coactivator recruitment.

    Journal of biochemistry·2026
    Same journal

    Chromatin dynamics and cellular energy homeostasis.

    Journal of biochemistry·2026
    Same journal

    Nutrigenomic Regulation of One-Carbon Metabolism and the Circadian Clock in Health and Disease.

    Journal of biochemistry·2026
    Same journal

    Time to rethink circadian rhythms beyond the transcriptome.

    Journal of biochemistry·2026
    Same journal

    BNIP3/NIX-dependent mitophagy: molecular mechanisms and physiological roles.

    Journal of biochemistry·2026
    Same journal

    Regulatory mechanism of heme-regulated inhibitor through autophosphorylation-driven activation and heme-induced deactivation.

    Journal of biochemistry·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Enzymology
    • Synthetic Chemistry

    Background:

    • Peptide synthesis is crucial for drug discovery and development.
    • Enzymatic methods offer potential advantages over traditional chemical synthesis.
    • Trypsin is a well-known protease with potential for synthetic applications.

    Purpose of the Study:

    • To investigate the feasibility of using trypsin for peptide synthesis.
    • To identify optimal conditions for trypsin-catalyzed peptide synthesis.
    • To explore the scope and limitations of trypsin in peptide bond formation.

    Main Methods:

    • Utilized N-acylated amino acid or peptide esters as donors.
    • Employed amino acid derivatives, peptides, or their derivatives as acceptors.

    Related Experiment Videos

  • Varied enzyme concentration, pH, substrate concentration, and nucleophile specificity.

    • Main Results:

    • Achieved near-quantitative peptide synthesis yields under optimized conditions.
    • Identified that higher enzyme and substrate concentrations, and alkaline pH favor synthesis over hydrolysis.
    • Found that hydrophobic or bulky amino acid residues at the P1' position enhance synthesis, while L-proline and D-amino acid residues are unfavorable.
    • Observed that product solubility influences yield, with lower solubility yielding higher product amounts.
    • Demonstrated the possibility of fragment condensation using peptide esters and peptides.

    Conclusions:

    • Trypsin can be effectively employed as a biocatalyst for peptide synthesis.
    • Optimal conditions include higher enzyme/substrate concentrations and alkaline pH.
    • Nucleophile specificity and product solubility are critical factors for successful synthesis.
    • Trypsin-catalyzed peptide synthesis presents a viable alternative for constructing peptide-based molecules.