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Updated: Aug 18, 2026

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
Published on: July 17, 2016
[Role of distant lymphoid cell interactions for the development of in vitro antibody formation]
Abstract:
In order to clarify the nature of the recently described intercellular local interactions which were shown to inhibit the antibody-forming cells (AFC) proliferation in vitro, a possibility of realization of this effect at a distance was studied. It was shown that the population with a great number of cells could inhibit in vitro at a distance an increase of the AFC in the population of cells separated from the former by a millipore filter, impermeable for the cells. The results were the same with the use of polymethylmetacrylate film (5 to 10 micrometer in thickness) impermeable for proteins with a molecular weight of 150000 daltons (125I-IgG-antibodies) and some ions (51CrO4), but permeable for other substances with low molecular weight.
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