[On the lipoperoxidase-isomerase-system from oat. Experiments for enzyme separation (author's transl)]

It was attempted to separate isomerase and lipoperoxidase activity from oat by means of gel and ion exchange chromatography and by isoelectric focusing. The molecular weight of the enzyme fraction tested was found to be of the order of 3 X 10(6) daltons. By ion exchange chromatography and isoelectric focusing the enzyme fraction with lipoperoxidase and isomerase activity could be separated into isoenzymes. These isoenzymes continued to exhibit both enzymatic activities. The lack of success in achieving of separation and the high molecular weight lead to the conclusion of the asistence of a close interrelation between the two enzymes or possibly of a bifunctional enzyme complex.