The primary structure of the gene encoding yeast ribosomal protein L16

As part of our studies on the molecular basis for the coordinate expression of ribosomal protein genes in yeast we analyzed the primary structure of the gene encoding protein L16 of the large ribosomal subunit including the flanking sequences. L16 turned out to be a ribosomal protein with a molecular mass of 22662 Da and a net charge of +12. Both the 5'- and the 3'-end of the L16 mRNA were mapped by primer extension and S1 nuclease analysis. In the DNA regions flanking the coding sequence several conserved elements are present that may be involved in transcription initiation or termination.